![]() ![]() We are catching up on orders of the 2lb and 1lb bags therefore please expect delays to our usual 3-5 working day guideline. Just some info I’ve learned.Disclaimer- Grow kits are made to order to ensure only the freshest quality products make their way to you. The key I’ve learned is using minimal amounts of spore solution and using injection ports or polyfill hole to knock up the jars, keeping contams from infiltrating the grains. I’ll be pressure cooking rye berries today and inoculating them tomorrow morning with multi spore inoculation. It’s about giving the mycelium the upper hand and allowing it to get a foothold over the grains. I PC them for 90mins The small amount of contams that are always present in syringes only gain control if i use to much spore solution, which causes bacteria to flourish, and mold spores germinate, and are sometimes present in the syringe or spawn (most likely the prior), if conditions are suited for them better than for the mycelium. I don’t believe any of my contamd jars came from dirty syringes, or dirty spawn. I have syringes I’ve made myself from prints so I can control how much spores I use and control proper sterile techniques. Using a small amount of spores is the easiest method for me to properly inoculate my grains, rye berries usually. I would rather inoculate jars with agar wedges or liquid cultures. I have a suspicion that before I used ships, opening the jar aloud contams to enter off the bat, so I purchased SHIPS from an online site to eliminate the problem. On my lids I’ve installed injection ports so the lid doesn’t need opened to inoculate. Too much spore solution can cause bacteria to flourish and offsets the H20 content in the jars. Using minimal spore solution and a self healing Injection port ups the chances of succeeding though. Using a Multi spore syringe on grains is a hit or miss thing in my experience. Finally, not sure what you mean by the sandwich technique with spores, but generally spores are added to the dish after it has cooled and solidified and often takes a number of transfers before you arrive at a pure culture. ![]() You can solve this by making sure you have enough volume of water in your pressure cooker to maintain consistent levels of heat as the cooker cools. ![]() As for the agar solution coming through the lid of the bottle- this is likely from “boil over” and it happens when the area around the agar cools down faster than the agar itself, which causes the hot agar solution to boil through the filter. You could definitely use the same method for 125 ml mason jar “petri dishes” just make sure you use a proper lid, not foil. Works every time! It is possible that the filter discs stayed wet and had some form of nutrition on them- a perfect environment for mold. Thanks for reaching out! I don’t use filter discs for the most part- i prefer to use poly stuffing that is pulled through a 1/4″ hole. Thanks, I know it’s a lot, but the site is awesome and I really think that growing on a budget should be rendered easier with those answer ! I wanted to use poly stuffing on both grain jars and small mason ‘petri’ with foil cover, but it did not seem to work … Any hint would be fine here, I just don’t get why ?įinally, I heard of using wild spore print via a sandwich of agar to purify it, but isn’t the agar pouring temperature way to high to mix in spores, and if not, would you care to detail how ? Using this technique, water flow trough the filter… I let you imagine the contamination level… I put them empty, sterilize, than I pour them in a SAB (by the way thanks for the bottle technique out here its awesome !). I think that the sterilization process is not adapted. However, I lost some transfers, do you have experience with this type of filters ? Should I allow the sterilization any longer, or is it normal for those to be partially digested in between batches ?įurthermore, I wanted to use 125 mL small mason pot in order to reduce the use of petri dish… I used the same filters and all the agar plate went catastrophic. I used filter disc made of cellulose and have 0.3 micron pores for my first generation jars, but the visible top became covered in mold !? … Apparently, the Oyster and Lion mane strains are doing just fine in rye grain. Sorry for the long post here, but I felt it was the best place to ask those 4 in 1 out. ![]()
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